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《现代肿瘤医学》[ISSN:1672-4992/CN:61-1415/R]

期数:

2019年20期

页码:

3597-3601

栏目:

论着（基础研究）

出版日期:

2019-09-08

文章信息/Info

Title:

Mechanisms of targeting VEGF gene combined with zoledronic acid on proliferation and migration of hepatocellular carcinoma cells

作者:

李自显¹;?徐 刚²;?王亚楠²;?杨文义³

1.濮阳市安阳地区医院消化内科，河南 安阳 455000;2.郑州大学第二附属医院消化内科，河南 郑州 450000;3.河南大学第一附属医院消化内科，河南 开封 475000

Author(s):

Li Zixian¹;?Xu Gang²;?Wang Yanan²;?Yang Wenyi³

1.Department of Digestive Medicine,Anyang District Hospital,Puyang City,Henan Anyang 455000,China;2.Department of Digestive Medicine,Second Affiliated Hospital of Zhengzhou University,Henan Zhengzhou 450000,China;3.Department of Digestive Medicine,First Affiliated Hospital of Henan University,Henan Kaifeng 475000,China.

关键词:

胃癌;?VEGF基因;?唑来膦酸;?细胞增殖;?STAT3信号通路

Keywords:

hepatoma carcinoma cell;?VEGF gene;?zoledronic acid;?cell proliferation;?STAT3 signaling pathway

分类号:

R735.2

DOI:

10.3969/j.issn.1672-4992.2019.20.011

文献标识码:

A

qq自动领红包软件:

目的：探讨靶向抑制VEGF基因联合唑来膦酸对胃癌细胞增殖、迁移及STAT3信号通路的影响。方法:采用LipofectamineTM2000将si-VEGF转染至胃癌BGC-823细胞中，以RT-PCR和Western blot 检测其转染效果。转染后，加入不同浓度的唑来膦酸共同培养48 h，MTT法检测si-VEGF联合唑来膦酸对BGC-823细胞增殖的影响，并检测唑来膦酸的IC50；将细胞随机分为si-NC组（转染阴性对照）、si-NC＋唑来膦酸组（转染阴性对照后，给予唑来膦酸处理）、si-VEGF组（转染si-VEGF）和si-VEGF+唑来膦酸组（转染si-VEGF后，给予唑来膦酸处理），Transwell小室实验检测细胞的侵袭和迁移能力，Western blot检测细胞中STAT3、p-STAT3和MMP-2蛋白的表达情况。结果:转染si-VEGF后成功下调BGC-823细胞中VEGF mRNA和蛋白的表达，si-VEGF联合唑来膦酸对BGC-823 细胞增殖的抑制作用明显高于唑来膦酸单独作用，且唑来膦酸的IC50为62.94 μmol/L。与si-NC组相比，si-NC+唑来膦酸组、si-VEGF组和si-VEGF+唑来膦酸组的侵袭细胞数及迁移细胞数均明显减少，且p-STAT3和MMP-2蛋白表达明显降低，而STAT3表达差异不明显。其中，si-VEGF+唑来膦酸组的作用效果明显高于si-NC+唑来膦酸组或si-VEGF组。结论:靶向抑制VEGF基因联合唑来膦酸能够协调抑制胃癌细胞增殖和迁移，其作用机制可能与抑制STAT3信号通路有关。

Abstract:

Objective:To investigate the effects of targeted inhibition of VEGF gene combined with zoledronic acid on proliferation,migration and STAT3 signaling in hepatocellular carcinoma cells.Methods:si-VEGF was transfected into BGC-823 cells by LipofectamineTM2000,and the transfection effects were detected by RT-PCR and Western blot.After transfection,different concentrations of zoledronic acid were used to co culture for 48 h.The effect of si-VEGF combined with zoledronic acid on the proliferation of BGC-823 cells was detected by MTT,and IC50 of zoledronic acid was detected.The cells were randomly divided into si-NC group (transfected negative control),si-NC+zoledronic acid group (after transfection of negative control,zoledronic acid treatment),si-VEGF group (transfected si-VEGF) and si-VEGF+zoledronic acid group (after transfection of si-VEGF,zoledronic acid treatment).The cell invasion and migration ability were detected by Transwell cell experiment,and the expression of STAT3,p-STAT3 and MMP-2 proteins in the cells were measured by Western blot.Results:After transfection of si-VEGF,the expression of VEGF mRNA and protein in BGC-823 cells was downregulated successfully.The inhibitory effect of si-VEGF combined with zoledronic acid on the proliferation of BGC-823 cells was significantly higher than that of zoledronic acid alone,and the IC50 of zoledronic acid was 62.94 μmol/L.Compared with the si-NC group,the numbers of invasive cells and migratory cells in the si-NC+zoledronic acid group,the si-VEGF group and the si-VEGF+zoledronic acid group were decreased obviously,and the expressions of p-STAT3 and MMP-2 protein were reduced obviously,but the differences of STAT3 expression were not obvious.Among them,the effect of si-VEGF+zoledronic acid group was significantly higher than that of si-NC+zoledronic acid group or si-VEGF group.Conclusion:The inhibition of VEGF gene and zoledronic acid can inhibit the proliferation and migration of hepatoma carcinoma cell,and the mechanism may be related to the inhibition of STAT3 signaling pathway.

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备注/Memo

备注/Memo:

河南省教育厅科研基金项目（编号：16B320003）

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更新日期/Last Update: 1900-01-01