|本期目录/Table of Contents|

结肠癌细胞中miR-145-5p、miR-143-3p的表达变化对多药耐药的影响

《现代肿瘤医学》[ISSN:1672-4992/CN:61-1415/R]

期数:
2019年20期
页码:
3592-3596
栏目:
论着(基础研究)
出版日期:
2019-09-08

文章信息/Info

Title:
Effects of expression changes of miR-145-5p and miR-143-3p in colon cancer cells on multidrug resistance
作者:
袁亚男;?杜梦楠;?黄艳洁;?许 倩;?郑纪宁
承德医学院病理学与病理生理学教研室,河北 承德 067000
Author(s):
Yuan Yanan;?Du Mengnan;?Huang Yanjie;?Xu Qian;?Zheng Jining
Pathology and Pathophysiology,Chengde Medical School,Hebei Chengde 067000,China.
关键词:
结肠癌;?多药耐药;?miR-145-5p;?miR-143-3p
Keywords:
colon cancer;?multidrug resistance;?miR-145-5p;?miR-143-3p
分类号:
R735.3+4
DOI:
10.3969/j.issn.1672-4992.2019.20.010
文献标识码:
A
qq自动领红包软件:
目的:探讨人结肠癌细胞中miR-145-5p、miR-143-3p的表达发生变化后对P-gp和MRP的影响。方法:采用Western Blot和qRT-PCR法检测人结肠癌细胞分别转染miR-145-5p mimics/inhibitor、miR-143-3p mimics/inhibitor后P-gp 和MRP的表达。结果:转染miR-145-5p mimics后P-gp和MRP的蛋白和基因表达均降低(P<0.05),转染miR-145-5p inhibitor后P-gp 和MRP的蛋白和基因表达均升高(P<0.05)。转染miR-145-5p mimics NC和miR-145-5p inhibitor NC后P-gp 和MRP的蛋白和基因表达无明显变化(P>0.05)。转染miR-143-3p mimics后P-gp和MRP的蛋白和基因表达均降低(P<0.05),miR-143-3p inhibitor后P-gp和MRP的蛋白和基因表达均升高(P<0.05)。转染miR-143-3p mimics NC和miR-143-3p inhibitor NC后P-gp 和MRP的蛋白和基因表达无明显变化(P>0.05)。结论:miR-145-5p及miR-143-3p对多药耐药蛋白P-gp、MRP具有调控作用,参与了结肠癌的多药耐药。
Abstract:
Objective:To investigate the effect of the expression changes of miR-145-5p and miR-143-3p on P-gp and MRP in colon cancer cells.Methods:The expressions of P-gp and MRP in colon cancer cells transfected with miR-145-5p mimics/inhibitor and miR-143-3p mimics/inhibitor were detected by Western Blot and qRT-PCR.Results:Protein and gene expression of P-gp and MRP were decreased after transfection with miR-145-5p mimics (P<0.05),and increased after transfection with miR-145-5p inhibitor (P<0.05).Protein and gene expression of P-gp and MRP were not significantly changed after transfection with miR-145-5p mimics NC and miR-145-5p inhibitor NC (P>0.05).Protein and gene expression of P-gp and MRP were decreased after transfection with miR-143-3p mimics (P<0.05), while protein and gene expression of P-gp and MRP were increased after transfection with miR-143-3p inhibitor (P<0.05).Protein and gene expression of P-gp and MRP showed no significant changes after transfection with miR-143-3p mimics NC and miR-143-3p inhibitor NC (P>0.05).Conclusion:miR-145-5p and miR-143-3p have regulatory effects on multidrug resistant proteins P-gp and MRP,and are involved in the occurrence,development and multidrug resistance of colon cancer.

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备注/Memo

备注/Memo:
河北省高校重点学科建设资助项目(编号:031412);承德医学院自然科学研究计划(编号:201707)
更新日期/Last Update: 1900-01-01