|本期目录/Table of Contents|

白藜芦醇上调BTG2表达抑制肝癌HepG2细胞增殖

《现代肿瘤医学》[ISSN:1672-4992/CN:61-1415/R]

期数:
2019年20期
页码:
3556-3560
栏目:
论着(基础研究)
出版日期:
2019-09-08

文章信息/Info

Title:
Resveratrol up-regulates the expression of BTG2 and inhibits the proliferation of hepatocellular carcinoma HepG2 cells
作者:
刘 兰;?饶智国;?张志敏;?付红星
解放军武汉总医院肿瘤内科,湖北 武汉 430070
Author(s):
Liu Lan;?Rao Zhiguo;?Zhang Zhimin;?Fu Hongxing
Department of Oncology,Wuhan General Hospital of Chinese People's Liberation Army,Hubei Wuhan 430070,China.
关键词:
白藜芦醇;?肝癌;?细胞增殖;?B细胞易位基因2
Keywords:
resveratrol;?hepatocellular carcinoma;?cell proliferation;?BTG2
分类号:
R735.7
DOI:
10.3969/j.issn.1672-4992.2019.20.003
文献标识码:
A
qq自动领红包软件:
目的:初步探讨白藜芦醇(resveratrol,Res)对肝癌HepG2细胞增殖及BTG2表达的影响。方法:不同浓度Res(终浓度分别为20、40、80 μmol/L)处理HepG2细胞,CCK-8法检测细胞增殖能力,流式细胞术检测各组细胞周期分布变化,RT-PCR、Western blot技术检测BTG2 mRNA和蛋白水平表达的变化。结果:Res可以显着抑制HepG2细胞的增殖,且呈浓度和时间依赖性(P<0.01),药物作用48 h后IC50为34.58 μmol/L。Res可使HepG2细胞周期阻滞在G2/M期;随着Res作用浓度的升高,抗增殖基因BTG2 mRNA和蛋白的表达逐渐增加(P<0.01)。结论:Res能够抑制肝癌HepG2细胞的增殖,对细胞周期具有阻滞效应且有浓度依赖性,其机制可能与其上调抗增殖基因BTG2表达相关。
Abstract:
Objective:To investigate the effect of resveratrol (Res) on the proliferation and expression of BTG2 in HepG2 cells.Methods:HepG2 cells were treated with different concentrations of Res (20,40,80 μmol/L),CCK-8 assay was used to detect cell proliferation.Flow cytometry was used to detect cell cycle distribution,RT-PCR and Western blot were used to detect the expression of BTG2 mRNA and protein.Results:Res could inhibit the proliferation of HepG2 cells in a concentration and time dependent manner (P<0.01),and IC50 value was 34.58 μmol/L after 48 hours of drug treatment,Res could block the cell cycle of HepG2 in G2/M phase,with the increase of Res concentration,the expression of BTG2 mRNA and protein were gradually increased (P<0.01).Conclusion:Res can inhibit the proliferation of HepG2 cells in a concentration-dependent manner,and its mechanism may be related to the up-regulation of BTG2 expression.

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备注/Memo

备注/Memo:
National Natural Science Foundation of China(No.81301631);国家自然科学基金资助项目(编号:81301631);湖北省卫生计生委科研基金(编号:WJ2018H0067)
更新日期/Last Update: 1900-01-01